Phylogenetlc Analysis of HN Gene of Eight Pigeon NDV Isolates in Guangxi.

Autor: Lu Bingxia, Liang Jiaxing, Duan Qunpeng, Chen Zhongwei, Jiang Dongfu, Lu Jingzhuan, Zhou Yingning, Bi Bingfen, He Ying, Qin Yibin, Li Bin, Su Qianlian, Zhao Wu
Předmět:
Zdroj: Animal Husbandry & Feed Science; Apr2018, Vol. 10 Issue 2, p136-138, 3p
Abstrakt: [Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease (ND). [Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR, sequenced and analyzed. The molecular evolution characteristics of HIV gene of pigeon NDV isolates in Guangxi was discussed. [Result] The nucleotide sequence length of HIV gene of the eight NDV isolates was 1 716 bp, encoding 571 amino acids. They belonged to virulent group C, and the gene length characteristic of HN gene accorded with virulent strain. Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb, ranging from 90.4% to 99.5%. Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Guangxi and the NDV isolates from Guangxi, Guangdong, Jilin, Liaoning, Yunnan and Heilongjiang during 2011 and 2013 was close. They were located in the same cladogram branch. [Conclusion] W e assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index