| Autor: |
Wang Liping, Han Xianjie, Wang Xiaobin, Gai Chunyun, Li Junwei, Yu Binhui, Shan Hu |
| Předmět: |
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| Zdroj: |
Animal Husbandry & Feed Science; Apr2018, Vol. 10 Issue 2, p115-125, 6p |
| Abstrakt: |
To evaluate the specific immune responses induced by recombinant Lactococcus lactis (L. lactis) which expresses porcine epidemic diarrhea virus (PEDV) SI protein through oral administration, the spike gene fragment of PEDV was amplified from PEDV SDLY strain to construct pMG36e-Sl recombinant plasmid. The recombinant plasmid was then electro-transferred into competent cells of L. lactis MG1363, to prepare the recombinant L. lactis expressing SI protein of PEDV. The expression of target protein was identified by SDS-PAGE and Western-blot. New Zealand White rabbits were orally administered with the recombinant strain; the antibody titer in intestinal mucosa and serum was detected by neutralizing test; and the specific IgG in serum was evaluated by indirect ELISA. The results showed that the recombinant L. lactis could effectively induce high level of IgG in serum and high level of mucosal immune antibody. The recombinant L. lactis is qualified to be a potential oral vaccine because it could successfully stimulate both humoral and mucosal immune responses against PEDV. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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