| Autor: |
Charney, Alan N., Egnor, Richard W., Henner, David, Rashid, Haroon, Cassai, Nicholas, Sidhu, Gurdip S. |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Cell Physiology; May2004, Vol. 286 Issue 5, pC1062-C1070, 9p, 1 Diagram, 3 Charts, 4 Graphs |
| Abstrakt: |
In rat ileum and colon, apical membrane C1¯/HCO3¯ exchange and net CI ¯absorption are stimulated by increases in Pco2 or [HCO3¯]. Because changes in Pco2 stimulate colonic Na+ absorption, in part, by modulating vesicular trafficking of the Na+/H+ exchanger type 3 isoform to and from the apical membrane, we examined whether changes in Pco2 affect net C1¯ absorption by modulating vesicular trafficking of the C1¯/HCO3¯ exchanger anion exchanger (AE)1. C1¯ transport across rat distal ileum and colon was measured in the Ussing chamber, and apical membrane protein biotinylation of these segments and Western blots of recovered proteins were performed. In colonic epithelial apical membranes, AE1 protein content was greater at Pco2 70 mmHg than at Pco2 21 mmHg but was not affected by pH changes in the absence of CO2. AE1 was internalized when Pco2 was reduced and exocytosed when Pco2 was increased, and both mucosal wortmannin and methazolamide inhibited exocytosis. Wortmannin also inhibited the increase in colonic C1¯ absorption caused by an increase in Pco2. Increases in Pco2 stimulated ileal C1¯ absorption, but wortmannin was without effect. Ileal epithelial apical membrane AE1 content was not affected by Pc02. We conclude that CO2 modulation of colonic, but not ileal, C1¯ absorption involves effects on vesicular trafficking of AE1. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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