| Autor: |
Hensgens, Charles M. H., Santos, Helena, Chenghong Zhang, Kruizinga, Wim H., Hansen, Theo A. |
| Předmět: |
|
| Zdroj: |
European Journal of Biochemistry; 12/1/96, Vol. 242 Issue 2, p327-331, 5p |
| Abstrakt: |
Under certain growth conditions the sulfate-reducing bacterium Desulfovibrio gigas forms electrondense granules in the cells which had been claimed to consist of a magnesium triphosphate). We observed granules after cultivation in media with a low Fe2+ or H4+ concentration and reinvestigated the nature of the electron-dense bodies. Energy-dispersive X-ray analysis of the granules in the cells showed that they contain large amount of P, Mg, and K. Gel electrophoresis and chromatographic analyses of isolated granules which had been dissolved in 20 mM EDTA, however, revealed discrepancies with commercially available polyphosphates. 31P-NMR spectra also lacked the peaks in the -22-ppm region which are characteristic for inner phosphates of polyphosphates confirming that the phosphocompound as isolated from the electron-dense bodies of D. gigas did not consist not consist of polyphosphates. Using multinuclear NMR spectroscopy we showed that the electron-dense bodies of Dl. Gigan contained a novel metabolite which was identified as α-glucose 1,2,3,4,6-pentakis (disphosphate). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
