| Autor: |
Koop, Ronald, Di Croce, Luciano, Beato, Miguel |
| Předmět: |
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| Zdroj: |
EMBO Journal; 2/1/2003, Vol. 22 Issue 3, p588-599, 12p |
| Abstrakt: |
Minichromosomes assembled on the mouse mammary tumor virus (MMTV) promoter in vitro exhibit positioned nucleosomes, one of which covers the binding sites for progesterone receptor (PR) and nuclear factor 1 (NF1). Incorporation of histone Hi into MMTV minichromosomes improves the stability of this nucleosome and decreases basal transcription from the MMTV promoter, as welt as its response to either PR or NF1. However, histone H1-containing minichromosomes display better PR binding and support a more efficient synergism between PR and NF1, leading to enhanced transcription initiation. A mutant MMTV promoter tacking positioned nucleosomes does not display enhanced transcriptional synergism in the presence of H1. Binding of PR leads to phosphorylation of H1, which leaves the promoter upon transcription initiation. Thus, Hi assumes a complex and dynamic rote in the regulation of the MMTV promoter. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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