| Autor: |
Sang-Ho Cha, Bandaranayaka-Mudiyanselage, Carey, Bandaranayaka-Mudiyanselage, Chandima B., Ajiththos, Dharani, Kyoung-Jin Yoon, Gibson, Kathleen A., Ji-Eun Yu, In-Soo Cho, Lee, Stephen S., Chung, Chungwon J. |
| Předmět: |
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| Zdroj: |
Korean Journal of Veterinary Research; 2018, Vol. 58 Issue 1, p9-16, 8p |
| Abstrakt: |
A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, CD3+CD4+, CD3+CD8+, CD3+CD4+CD8+ T-lymphocytes, and PRRSVspecific interferon (IFN)-γ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages (CD172α+PRRSV-N+ PAM) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of CD172α+PRRSV-N+ PAM (p < 0.05) as well as with macroscopic lung lesion (p < 0.01). Plasma and tissue viral loads had significant (p < 0.05) negative correlations with CD3+CD4+CD8+ T-lymphocyte percentage in PBMC. Frequencies of CD3+CD8+ and CD3+CD4+ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p = 0.04) and lung (p = 0.002) viral loads. IFN-γ-secreting T-lymphocytes frequency had a significant negative correlation with gross lung lesion severity (p = 0.002). However, neutralizing antibody titers had no significant negative correlation (p > 0.1) with infection parameters. The results indicate that T-lymphocytes contribute to controlling PRRSV replication in young piglets born after in-utero infection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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