| Autor: |
Sund, Maim, Ylönen, Riikka, Tuomisto, Anne, Sormunen, Raija, Tahkola, Jenni, Kvist, Ari-Pekka, Kontusaari, Sirpa, Autio-Harmainen, Helena, Pihlajaniemi, Taina |
| Předmět: |
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| Zdroj: |
EMBO Journal; 9/15/2001, Vol. 20 Issue 18, p5153-5164, 12p |
| Abstrakt: |
Type XIII collagen is a type II transmembrane protein found at sites of cell adhesion. Transgenic mouse lines were generated by microinjection of a DNA construct directing the synthesis of truncated α(XIII) chains. Shortened α1(XIII) chains were synthesized by fibroblasts from mutant mice, and the tack of intracellular accumulation in immunofluorescent staining of tissues suggested that the mutant molecules were expressed on the cell surface. Transgene expression led to fetal lethality in offspring from heterozygous mating with two distinct phenotypes. The early phenotype fetuses were aborted by day 10.5 of development due to a tack of fusion of the chorionic and allantoic membranes. The late phenotype fetuses were aborted by day 13.5 of development and displayed a weak heartbeat, defects of the adherence junctions in the heart with detachment of myofilaments and abnormal staining for the adherence junction component cadherin. Decreased microvessel formation was observed in certhin regions of the fetus and the placenta. These results indicate that type XIII collagen has an important role in certain adhesive interactions that are necessary for normal development. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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