| Abstrakt: |
Seeds of Silene coeli-rosa L. were germinated and grown at 20°C in short days of 8 h light from fluorescent and tungsten (F+T) bulbs and 16 h darkness for 28 days (day 0). At 1700 h of day 0, the plants were exposed to 16 h light from T (LD) followed by 8 b F+T, or the same treatment interrupted at 1700 h of day 0 by 20 or 60 min darkness. Plants were exposed to tritiated (methyI-[³H])-thymidine for 2 h (1645- 1845 h) and sampled every 2 h for 24 h. The cell cycle (percentage labelled mitoses method), and changes in cell number were measured in the shoot apical meristems. The cell cycle in the LD, 20 and 60 mm dark-interrupted LD (diLD) treatments was 10, 11 and 13 h, respectively. Mean cell generation times were ca 3-5 h longer, suggesting that the shorter cell cycles were transient. The proportions of cells with 2C or 4C amounts of nuclear DNA, indicated that imposition of darkness resulted in a progressive lengthening of G1 from about 3 h in the LD to 7 h in the 64 min diLD treatment. Conversely, G2 shortened from about 4 h in the LD to 3 h in the 60 mm diLD treatment. Measurements of labelling index indicated that S-phase was about ii to 2 h in each treatment. The data are discussed in relation to the known inhibitory effect of the diLD treatments on flowering. [ABSTRACT FROM AUTHOR] |
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