| Autor: |
Colling, Axel, Lunt, Ross, Bergfeld, Jemma, McNabb, Leanne, Halpin, Kim, Juzva, Susan, Newberry, Kim, Morrissy, Chris, Loomes, Cameron, Warner, Simone, Diallo, Ibrahim, Kirkland, Peter, Broder, Christopher C., Carlile, Gemma, Mai Hlaing Loh, Waugh, Caryll, Wright, Lynda, Watson, James, Eagles, Debbie, Zuelke, Kurt |
| Předmět: |
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| Zdroj: |
Journal of Veterinary Diagnostic Investigation; May2018, Vol. 30 Issue 3, p362-369, 8p |
| Abstrakt: |
Obtaining statistically sound numbers of sera from Hendra virus (HeV)-infected horses is problematic because affected individuals usually die or are euthanized before developing a serum antibody response. As a consequence, test validation becomes a challenge. Our approach is an extension of OIE principles for provisional recognition and included 7 validation panels tested across multiple laboratories that provided estimates for test performance characteristics. At a 0.4 S/P cutoff, 16 of 19 sera from HeV-infected horses gave positive results in the HeV soluble G, indirect ELISA (HeVsG iELISA; DSe 84.2% [95% CI: 60.4-96.6%]); 463 of 477 non-infected horse sera tested negative (DSp 97.1% [95% CI: 95.1-98.4%]). The HeVsG iELISA eliminated almost all false-positive results from the previously used HeV iELISA, with marginally decreased relative sensitivity. Assay robustness was evaluated in inter-laboratory and proficiency testing panels. The HeVsG iELISA is considered to be fit for purpose for serosurveillance and international movement of horses when virus neutralization is used for follow-up testing of positive or inconclusive serum samples. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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