Autor: |
Yanagisawa, Naoki, Higashiyama, Tetsuya |
Předmět: |
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Zdroj: |
Biomicrofluidics; 2018, Vol. 12 Issue 2, pN.PAG-N.PAG, 8p |
Abstrakt: |
We present a semi-in vitro chemotropism assay that can be used to evaluate the chemoattractant effect of diffusible plant signaling molecules on growing pollen tubes. We constructed an array of microslit channels in a microfluidic device that prevented the passage of randomly growing pollen tubes but permitted ones that are responsive to the chemoattractant. Depending on the microslit channel size, 80%–100% of the randomly growing Torenia fournieri pollen tubes were excluded from reaching the source of the attractant. Thus, the selection of pollen tubes that are capable of responding to chemoattractants from a mixed population can be realized using this platform. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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