| Autor: |
K. von Schwartzenberg, W. Schultze, H. Kassner |
| Předmět: |
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| Zdroj: |
Plant Cell Reports; May2004, Vol. 22 Issue 10, p780-786, 7p |
| Abstrakt: |
The presence of the tetracyclic diterpene 16a-hydroxykaurane (16a-hydroxy- ent-kaurane, C 20H 34O, CAS 5524?17?4) was detected in sterile cell cultures of the moss Physcomitrella patens (Hedw.) B.S.G. using gas chromatography and mass spectrometry. 16a-hydroxykaurane was found to be a major lipid compound in P. patens, with an estimated intracellular concentration of up to 0.84 mmol/l and an extracellular concentration of up to 9.3 µmol/l. The overall content of 16a-hydroxykaurane (in milligrams) produced per culture reached 0.37-fold that of chlorophyll a+ b. In agar cultures with low air exchange, 16a-hydroxykaurane forms needle-like crystals on tissue and on the inner surface of the culture vessels, indicating that it is being released into the atmosphere. Solid phase microextraction confirmed the air-bound release of 16a-hydroxykaurane. To our knowledge this is the first report on the release of a plant-derived tetracyclic diterpene into the air. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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