| Autor: |
Hanke, Till, Bartmann, Peter, Hennecke, Hauke, Kosakowski, Heinz M., Jaenicke, Rainer, Holler, Eggebard, Böck, August |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; Apr74 Part 2, Vol. 43 Issue 3, p601-607, 7p |
| Abstrakt: |
The molecular weight, of L-phenylalanyl-tRNA synthetase was redetermined. A new value of 267000 was obtained by high-speed analytical ultracentrifugation and Sephadex G-200 gel chromatography. The value is in contrast to tile molecular weight of 180000 determined previously; From tile molecular weight 267000 and the sedimentation coefficient s20,w = 8.6 S the ratio ƒ/ƒ0 = 1.67 was calculated. The magnitude of ƒ/ƒ0 is in accord with tile molecular weight 183 000 obtained from sucrose gradient centrifugation. The results indicate that the enzyme is highly hydrated and/or assumes the shape of a rod or a disc. The subunit composition was reinvestigated by sodium dodecylsulfate gel electrophoresis, guanidinium chloride gel filtration and by reactivation experiments of pH-2 inactivated enzyme. The results are consistent with a subunit structure of the α2β2 type. Molecular weights of the subunits are 39000 and 94000, respectively. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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