| Autor: |
Hu, Xiaojing, Liu, Junjie, Zhu, Ping, Wei, Dan, Jin, Jian, Liu, Xiaobing, Wang, Guanghua |
| Předmět: |
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| Zdroj: |
Journal of Soils & Sediments: Protection, Risk Assessment, & Remediation; May2018, Vol. 18 Issue 5, p2053-2062, 10p |
| Abstrakt: |
Purpose: Although biological nitrogen fixation is an important nitrogen source for agroecosystem, the knowledge of their response to long-term fertilization under neutral soil is limited. The aims of this study were to explore the effects of different fertilization treatments on diversity and compositions of diazotrophic community in a neutral black soil in northeast China.Materials and methods: The long-term fertilization experiment under monoculture maize was established in a neutral black soil since 1979. Soil samples from four treatments were collected in September 2014: non-fertilization (NoF), chemical fertilization (CF), manure fertilization (M), and chemical fertilization plus manure (CFM). qPCR and Illumina MiSeq sequencing were used to assess the abundance and compositions of diazotrophic community targeting nifH gene.Results and discussion: M treatment significantly increased the nifH gene abundance, but inverse trends were found in the CF and CFM treatments when compared with NoF. The diazotrophic community was overwhelmingly dominated by Alphaproteobacteria with relative higher abundance in manure-added treatments. Principal coordinate analysis (PCoA) indicated that the total diazotrophic community was separated into two groups with and without manure addition, and soil available P was the most influential factor on the changes of diazotrophic community.Conclusions: Our results suggested that soil nutrients rather than soil pH were the main influential factors on the changes of diazotrophic communities and manure addition had a greater effect than chemical fertilization on diazotrophic community structure in neutral black soil. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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