PCR BASED DETECTION OF S. AUREUS CLONAL COMPLEX 398 IN CHICKEN AND MUTTON MARKETED IN CHENNAI, INDIA.

Autor: Ruban, S. Wilfred, Babu, R. Narendra, Porteen, K., Senthilkumar, T. M. A., Raja, P., Kumarasamy, P., Abraham, Robinson J. J., Rao, V. Appa
Předmět:
Zdroj: Journal of Experimental Zoology India; 2018, Vol. 21 Issue 1, p217-220, 4p
Abstrakt: Differentiation of S. aureus isolates from humans and livestock is imperative to identify the origin of the isolates in meat and meat products and hence discrimination of different biotypes of S. aureus is of more importance. S. aureus Clonal Complex (CC) 398 remains the most commonly identified type of Livestock associated Methicillin resistant S. aureus worldwide. Hence, the present study was carried out to evaluate the prevalence of S. aureus Clonal Complex 398 in chicken and mutton marketed in in retail outlets of Chennai, India. A total of 40 chicken isolates (27 MRSA and 13 MSSA)and 40 mutton isolates (16 MRSA and 24 MSSA) were screened for the presence of S. aureus CC398 by PCR. It was observed thatof the 40 chicken isolates, 28 isolates belonged to CC398 (70%) and 21 of the 40 mutton (52.5%) isolates belonged to CC398. It was evident that the overall prevalence of S. aureus CC398 in retail mutton and Chicken samples was 61.25 per cent. The high prevalence of CC398 indicates that the major source of contamination of retail meat was the animal skin and faecal materials since it has been well documented that this sequence type has been most predominant in livestock. In the present study it was also observed that of the CC398 was prevalent both in Methicillin resistant isolates (28/43-65.12%) and in Methicillin sensitive isolates (21/37-56.76%). The results of the present study indicates that S. aureus CC398 is prevalent in isolates from retail meat, indicating their potential zoonotic importance to human population especially meat handlers. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index