Microculture of single protoplasts of Brassica napus.

Autor: Spangenberg, G., Koop, H.-U., Liebter, R., Schweiger, H. C.
Předmět:
Zdroj: Physiologia Plantarum; Jan1986, Vol. 66 Issue 1, p1-8, 8p
Abstrakt: Protoplasts of Brassica napus L.. were cultured individually in a microdroplet system using a synthetic medium with survival rates of more than 70% and division frequencies of up to 65%. Microcallus formation occurred at frequencies of up to 50%. Factors affecting the survival and division of individually cultured protoplasts, such as composition and volume of culture medium, pH, buffering system, osmolarity and genotype, were analyzed. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index