Application of DEPArrayTM technology for the isolation of white blood cells from cell mixtures in chimerism analysis.

Autor: Anslinger, K., Bayer, B., von Máriássy, D.
Zdroj: Rechtsmedizin; Apr2018, Vol. 28 Issue 2, p134-137, 4p
Abstrakt: After allogeneic bone marrow or blood stem cell transplantation, STR profiling is often used for monitoring chimerisms. For the evaluation reference samples taken from the patient before the transplantation and/or taken from the donor are needed. In the case described here, no reference samples were available. Reporting the chimerism based on posttransplantation samples did not yield conclusive results; therefore, white blood cells and epithelial cells obtained from a buccal swab taken from the patient were separated using DEPArrayTM technology and STR profiling was carried out resulting in distinct reference profiles. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index