| Autor: |
Scandolera, Amandine, Hubert, Jane, Humeau, Anne, Lambert, Carole, De Bizemont, Audrey, Winkel, Chris, Kaouas, Abdelmajid, Renault, Jean-Hugues, Nuzillard, Jean-Marc, Reynaud, Romain |
| Zdroj: |
Marine Drugs; Mar2018, Vol. 16 Issue 3, p96, 15p |
| Abstrakt: |
The aimof the present study was to investigate the neuro-soothing activity of a water-soluble hydrolysate obtained fromthe redmicroalgae Rhodosorus marinus Geitler (Stylonemataceae). Transcriptomic analysis performed on ≈100 genes related to skin biological functions firstly revealed that the crude Rhodosorus marinus extract was able to significantly negatively modulate specific genes involved in pro-inflammation (interleukin 1α encoding gene, IL1A) and pain detection related to tissue inflammation (nerve growth factor NGF and its receptor NGFR). An in vitromodel of normal human keratinocytes was then used to evaluate the ability of theRhodosorusmarinus extract to control the release of neuro-inflammation mediators under phorbol myristate acetate (PMA)-induced inflammatory conditions. The extract incorporated at 1%and 3%significantly inhibited the release of IL-1α andNGF secretion. These resultswere confirmed in a co-culture system of reconstructed human epithelium and normal human epidermal keratinocytes on which a cream formulated with the Rhodosorus marinus extract at 1% and 3% was topically applied after systemic induction of neuro-inflammation. Finally, an in vitro model of normal human astrocytes was developed for the evaluation of transient receptor potential vanilloid 1 (TRPV1) receptormodulation,mimicking pain sensing related to neuro-inflammation as observed in sensitive skins. Treatment with the Rhodosorus marinus extract at 1% and 3% significantly decreased PMA-mediated TRPV1 over-expression. In parallel with these biological experiments, the crude Rhodosorus marinus extract was fractionated by centrifugal partition chromatography (CPC) and chemically profiled by a recently developed 13CNMR-based dereplicationmethod. The CPC-generated fractions aswell as puremetaboliteswere tested again in vitro in an attempt to identify the biologically active constituents involved in the neuro-soothing activity of theRhodosorusmarinus extract. Two activemolecules, namely, -aminobutyric acid (GABA) and its structural derivative GABA-alanine, demonstrated a strong capacity to positively regulate skin sensitization mechanisms related to the TRPV1 receptors under PMA-induced inflammatory conditions, therefore providing interesting perspectives for the treatment of sensitive skins, atopia, dermatitis, or psoriasis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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