| Autor: |
Omran, Rabab, AL-Fatlawi, Ilham N. A. |
| Předmět: |
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| Zdroj: |
Pakistan Journal of Biotechnology; 2017, Vol. 14 Issue 4, p683-687, 5p |
| Abstrakt: |
Objective: There are numerous procedures accessible to extract nucleic acid from many cellular sources such as whole blood samples and choosing an appropriate protocol still require consideration of numerous different factors, and perform more researches. The aim of this paper was improved special protocol for DNA extraction from frozen blood samples which is fast, inexpensive and reliable for downstream molecular applications. Methods: Blood samples were collected from 25 persons (from Hilah City-Iraq) for extracting genomic DNA using manual method (by using 500µL blood sample volume) and Geneaid purification kit as a control by using 200µL blood sample volume. The concentrations and purity of DNA extracts were estimated by NanoDrop spectrophotometer. The presence of enzyme inhibitors in the two extracts was tested via polymerase chain reaction (PCR) by detection of the SOD2 gene. Results: The DNA extracts from frozen blood samples using a manual procedure had acceptable concentration and purity were 63.08±24.28 ng/µL and 1.39±0.16, respectively and the final volume of DNA extract about 300 µL, compared to control method (Geneaid purification kit) (86.91±32.97 ng/µl, 1.74±0.13, and 100 µL respectively). The DNA extracts were free enzyme inhibitors, which confirmed by PCR. Conclusion: Based on the experimental results the manual method could be recommended as a rapid and inexpensive Lab procedure for DNA extraction from either fresh or frozen blood samples. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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