| Autor: |
Vafina, Gulnaz, Zainutdinova, Elmira, Bulatov, Emil, Filimonova, Maria N. |
| Předmět: |
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| Zdroj: |
Frontiers in Pharmacology; 2/16/2018, p1-N.PAG, 8p |
| Abstrakt: |
One of the approaches to effective airway cleansing is the degradation of DNA into smaller fragments. For this purpose PulmozymeR is used with high efficacy because it contains recombinant DNase I as its active component. The aim of the study was to comparatively analyze DNase activity of PulmozymeR and the nuclease from gram-negative bacteria Serratia marcescens, because at optimal conditions the catalytic efficiency of the nuclease is much higher than the efficiency of DNase I. Highly polymerized DNA and purulent-mucous sputum were used as substrates. The examination showed that both S. marcescens nuclease and PulmozymeR hydrolyzed DNA in sputum. Also S. marcescens nuclease was found capable of hydrolyzing DNA in conditions that are standard for PulmozymeR and suitable for its therapeutic application. For manifesting the similar hydrolytic activity the nuclease amount in the assay mixture containing highly polymerized DNA or the sonicated sputum and NaCl together with calcium- or magnesium- cations can be about 10- time lower than that of the recombinant DNase I. In the presence of magnesium cations the DNase activity of both S. marcescens nuclease and PulmozymeR was higher than in the presence of calcium cations. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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