| Autor: |
عظیمی, آرین, مظاهري, زهره, موحدین, منصوره |
| Zdroj: |
Pathobiology Research; Autumn2017, Vol. 20 Issue 3, p49-60, 12p |
| Abstrakt: |
Objective: Pluripotent stem cells derived from testis are a new, unlimited source for cell therapy in regenerative medicine. Recently, studies show that spermatogonial stem cells can form embryonic stem-like cells (ES-like cells) in vitro. New procedures such as low intensity ultrasound (LIUS) can have positive effects on cell growth and differentiation. However, the effect of LIUS stimulation on ES-like cells has not been explored. In this study we investigate the effects of LIUS on colonization of ES-like cells. Methods: Initially, we isolated SSCs from neonatal mice. The spermatogonial and Sertoli cells were cultured together in DMEM/F12 supplemented with 15% fetal bovine serum (FBS) and leukemia inhibitory factor (LIF). ES-like cells were stimulated by LIUS at intensity doses of 200 millwatt/square centimeter (mW/cm2) over 5 days. Characteristics of the isolated cells were confirmed by immunocytochemistry with Sox2 and SSEA-1 protein for ES-like cells. We also investigated colonization features in the ES-like cells. Results: After 21 days, we observed there was a significant increase in diameter and number of colonies in the 200 mW/cm2 group compared to the control group (p≤0.05). Pluripotency proteins, ES-like cell marker Sox2, and SSEA-1 expressed in the ES-like cells. Conclusion: LIUS treatment can be an efficient, cost-effective method to improve colonization of ES-like cells during culture. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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