POF Immunosensor with Antibodies Immobilization for Escherichia coli.

Autor: Lopes, R. N., Rodrigues, D. M. C., Allil, A. S., Allil, R. C. S. B., Werneck, M. M.
Předmět:
Zdroj: International Conference on POF Proceedings; 2017, p137-140, 4p
Abstrakt: The immunosensors are characterized by antibodies as biological recognition element, responsible for the specificity of the biosensor. For the development of immunsensors based on plastic optical fiber (POF) technology, it is necessary the amination of the fiber core surface, which is made of polymethylmethacrylate (PMMA). In this study we compared four functionalization protocols of PMMA and immobilization of antibodies on the surface of the immunosensor development for the detection of Escherichia coli. Tests with bacterial suspensions in 108 CFU/mL concentrations were carried out to evaluate the response of the immunosensors functionalization with different protocols. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index