Knockdown of microRNA-29a regulates the expression of apoptosis-related genes in MCF-7 breast carcinoma cells.

Autor: Khamisipour, Gholamreza, Mansourabadi, Elham, Naeimi, Behrouz, Moazzeni, Ali, Tahmasebi, Rahim, Hasanpour, Mojtaba, Mohammadi, Majid Mosahebi, Mansourabadi, Zahra, Shamsian, Shakib
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Zdroj: Molecular & Clinical Oncology; 2018, Vol. 8 Issue 2, p362-369, 8p
Abstrakt: MicroRNA (miR), as non-coding small RNA, are key regulators of cancer-related biological cell processes and contribute to tumor growth through regulation of groups of pro- and anti-apoptotic genes. The present study aimed to investigate the effects of miR-29a on the expression of genes involved in apoptosis, including p21, B-cell lymphoma 2 (BCL-2), p53 and survivin. The MCF-7 breast cancer cell line was transfected with anti-miR-29a and treated with Taxol in subdivided treatment groups including: Scramble; anti-miR-29a; anti-miR-29a + Taxol; Taxol; and control. Expression levels of p21, BCL-2, p53 and survivin were evaluated using reverse transcription-quantitative polymerase chain reaction. miR-29a knockdown resulted in p21 and p53 upregulation and a decrease in survivin expression. These results indicated that miR-29a inhibition regulates apoptosis. The present data suggested that miR-29a inhibition may be a promising strategy for the induction of apoptosis of tumor cells. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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