POLYMORPHISM OF β-LACTOGLOBULIN IN PRAMENKA SHEEP BREED IN BOSNIA AND HERZEGOVINA.

Autor: Rustempašić, A., Dokso, A., ZeČević, E., Hodžić, A., Hrković-Porobija, A., Sarić, Z., Miskoska-Milevska, E., Popovski, Z., Brka, M.
Předmět:
Zdroj: JAPS: Journal of Animal & Plant Sciences; Jan2018, Vol. 28 Issue 1, p337-340, 4p, 2 Charts
Abstrakt: The aim of this study was to determinate allele frequency β-lactoglobulin in Pramenka breed sheep in Bosnia and Herzegovina. The DNA was extracted from blood of 283 sheep, collected from four flocks around Sarajevo, Bosnia and Herzegovina. The gene β-lactoglobulin was amplified using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) technique. Two genetic variants (A and B) and three genotypes (AA, AB and BB) of β-lactoglobulin have been identified. Genotype frequencies of β-lactoglobulin were with the following values: AA (0.325), AB (0.445), and BB (0.229), while the frequencies of allele A and B were 0.547 and 0.453 respectively. The highest frequency of genotype AA is present in the location Treskavica (38%), genotype AB in the location Vlašić (52%), while the genotype BB is mostly present in location Bjelašnica (29%). The population of Pramenka breed sheep was in Hardy-Weinberg equilibrium. It is expected that in a future the programs in sheep breeding in Bosnia and Herzegovina certainly would take into account the genetic variants of β-lactoglobulin that could contribute to better production and economic benefit. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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