Effect of Aspirin and ibuprofen either alone or in combination on gastric mucosa and bleeding time and on serum prostaglandin E and thromboxane A levels in the anaesthetized rats in vivo.

Autor: Bastaki, Salim M. A., Padol, Ireneusz T., Amir, Naheed, Hunt, Richard H.
Zdroj: Molecular & Cellular Biochemistry; Jan2018, Vol. 438 Issue 1/2, p25-34, 10p
Abstrakt: There is much evidence that a combination of ibuprofen (IBU) and Aspirin (ASA) can antagonize the irreversible inhibition of platelet function. This study was designed to investigate the degree of gastric damage, bleeding time (BT) and fluctuations in the serum levels of prostaglandin E (PGE) and thromboxane A (TXA) after oral administration of ASA (200 mg/kg) and IBU (50 mg/kg) either alone or in combination in rats in vivo. The stomach was assessed for any damage either after 6 h, 18 h or 6 days and carboxymethylcellulose (1% CMC) served as a vehicle and control. ELISA was used to measure TXA and PGE in serum. Bleeding time was assessed using tail blood. The results show that ASA and IBU either alone or in combination can cause gastric ulceration in 25-100% of the rats at 6 and 18 h. In contrast, gastric ulceration was seen in 50% of rats with a combination of ASA given before IBU only after 6 days of oral administration. BT was unaffected either by ASA or IBU when administered alone except after 18 h for IBU. In contrast, BT was significantly reduced when IBU was administered before ASA after 18 h and 6 days ( P < 0.001). Serum PGE levels decreased significantly after ASA administered either alone or in combination with IBU for 6 h, 18 h and 6 days ( P < 0.05). Serum TXA levels were significantly reduced after 6 h, 18 h and 6 days following ASA and IBU administration except for IBU alone which caused a significant increase in serum TXA 6 days after its administration ( P < 0.01). It can be concluded that ASA and IBU administered either alone or in combination can cause gastric ulcers in the rat stomach after 6 h and 18 h, but less severe after 6 days. IBU either alone or in combination with ASA reduced BT only after 18 h and 6 days of administration. Together, the results show that gastric ulceration correlated well with the inhibition of serum PGE and TXA levels. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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