| Autor: |
David, Hélène, Domon, Jean-Marc, Savy, Colette, Miannay, Nicole, Sulmont, Gérard, Dargent, Robert, David, Alain |
| Předmět: |
|
| Zdroj: |
Physiologia Plantarum; Jun92, Vol. 85 Issue 2, p301-307, 7p |
| Abstrakt: |
In the genus Prunus, so far, somatic embryogenesis has not been reported either from cell suspensions or from their protoplast-derived cells. Rhizogenic cell suspensions of Prunus avium L., initiated from adventitious roots developed from cotyledon-derived callus of mature zygotic embryos, have been subcultured for more than one year without losing their morphogenic potential. A yield of 8 × 105 protoplasts ml-1 of packed cells with a viability of 98% has been routinely obtained. Optimum cell division frequency (around 2.5% at day 10 and 4–6% at day 15) occurs in agarose lenses, with Murashige and Skoog (1962. Physiol. Plant 15: 476–497) -based medium supplemented with 5 µM naphthalene acetic acid, 1 µM benzyladenine and 0.25 µM zeatin. Colony formation has been achieved after 35 days with a plating efficiency of 3–4%. Cell suspensions have been initiated from protoplast-derived callus. While the older cell cultures express a rhizogenic response, the younger ones contain early stages of somatic embryo development. Ultrastructural examination confirms the polarization of these structures. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text