| Autor: |
李扬, 张延辉, 王云枫, 魏鑫, 关利新, 关悦 |
| Předmět: |
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| Zdroj: |
Chinese Journal of Osteoporosis; Aug2017, Vol. 23 Issue 8, p1030-1033, 4p |
| Abstrakt: |
Objective To investigate the effect of Salvia miltiorrhiza extract on the proliferation of bone marrow mesenchymal stem cells ( BMMSCs) and osteogenic differentiation. Methods BMMSCs were cultured using whole bone marrow cells inoculation method and adherent purification. Salvia miltiorrhiza extract (SME) , in the concentrations of 0. 2, 0.4, 0. 8, 1.6 mg/ mL and 3. 2 mg/mL, was added to bone marrow mesenchymal stem cells and its proliferation effect on bone marrow mesenchymal stem cell were observed. Differentiation of bone marrow mesenchymal stem cells into osteoblasts had been induced by Salvia miltiorrhiza extract (0.4, 0.8 mg/mL and 3.2 mg/mL) for 21 days. ALP kit was used to determine the activity of ALP in osteoblasts and Alizarin red staining was used to observe calcified nodules in osteoblasts. The content of osteoblast related protein BMP-2 and Runx-2 was determined using Western blotting. Results Different concentrations of Salvia miltiorrhiza extract could promote the proliferation of bone marrow mesenchymal stem cells, and the strongest effect was observed for the concentration of 0. 8 mg/mL. ALP assay showed that Salvia miltiorrhiza extract in the concentrations of 0.4，0.8 mg/mL and 3.2 mg/mL could improve ALP activity and the concentration of 0. 8 mg/mL had the highest ALP activity. Alizarin red staining showed that Salvia miltiorrhiza extract in the concentrations of 0. 4, 0.8 mg/mL and 3.2 mg/mL could promote bone cell calcification, and the concentration of 0. 8 mg/mL had the highest number of calcified nodules. Western blotting results showed that 0. 8 mg/mL Salvia miltiorrhiza extract could promote the expression of osteoblast differentiation related protein BMP-2 and Runx. Conclusion Salvia miltiorrhiza extract can promote the proliferation of bone marrow mesenchymal stem cells and osteogenic differentiation in rats. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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