| Autor: |
Klinck, Roscoe, Liquier, Jean, Taillandier, Eliane, Gouyette, Catherine, Huynh-Dinh, Tam, Guittet, Eric |
| Předmět: |
|
| Zdroj: |
European Journal of Biochemistry; 10/15/95, Vol. 233 Issue 2, p544-553, 10p |
| Abstrakt: |
A chemically synthesized 29-base RNA oligomer, designed to fold to form an intramolecular triple helix at acid pH, has been studied by NMR spectroscopy. The molecule consisted of seven U·A·U or C+·G·C base triples joined by two pyrimidine telra-loops. The fold was such that the third strand was Hoogsteen base-paired in the major groove of a Watson-Crick paired double helix. The nature and size of the molecule required the use of an assignment strategy using two- and three-dimensional homonuclear methods, complemented by a natural abundance 13C correlation experiment. The assignment of the majority of the exchangeable and non-exchangeable resonances is presented. The data suggest a C3′-endo sugar puckering for all the nucleotides involved in base triples. A preliminary structural model consistent with the NMR data is presented. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
