| Abstrakt: |
The isoenzymatic pattern of superoxide dismutase (SOD; EC 1.15.1.1) was studied in the symbiosis of Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe-Trifolium pratense L. A Cu,Zn-SOD (Mr 40 500) was found in spores of G. mosseae, while one Mn-SOD (Mn-SOD I) and two Cu,Zn-SODS (Cu,Zn-SOD I and Cu,Zn-SOD II) were present in both roots and leaves of T. pratense. Molecular masses for Cu,Zn-SOD I and Cu,Zn-SOD II were 31 000 and 34 300, respectively. However, when T. pratense and G. mosseae were associated, mycorrhizal roots showed two new isozymes, Mn-SOD II and mycCu,Zn-SOD, which have relative molecular masses of 37 800 and 33 300, respectively. The mycCu,Zn-SOD was found to be specific for this association, whereas Mn-SOD II was also present in nodules of Rhizobium-T. pratense. Results suggest that both enzymes are induced in the T. pratense roots in response to invasion by mycorrhizal fungi, perhaps as a result of an increase in the generation of O2- radicals in plant roots. [ABSTRACT FROM AUTHOR] |
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