| Autor: |
Good, Katrina V., Martínez de Paz, Alexia, Tyagi, Monica, Cheema, Manjinder S., Thambirajah, Anita A., Gretzinger, Taylor L., Stefanelli, Gilda, Chow, Robert L., Krupke, Oliver, Hendzel, Michael, Missiaen, Kristal, Underhill, Alan, Landsberger, Nicoletta, Ausió, Juan |
| Zdroj: |
Epigenetics; 2017, Vol. 12 Issue 11, p934-944, 11p |
| Abstrakt: |
MeCP2 binds to methylated DNA in a chromatin context and has an important role in cancer and brain development and function. Histone deacetylase (HDAC) inhibitors are currently being used to palliate many cancer and neurological disorders. Yet, the molecular mechanisms involved are not well known for the most part and, in particular, the relationship between histone acetylation and MeCP2 is not well understood. In this paper, we study the effect of the HDAC inhibitor trichostatin A (TSA) on MeCP2, a protein whose dysregulation plays an important role in these diseases. We find that treatment of cells with TSA decreases the phosphorylation state of this protein and appears to result in a higher MeCP2 chromatin binding affinity. Yet, the binding dynamics with which the protein binds to DNA appear not to be significantly affected despite the chromatin reorganization resulting from the high levels of acetylation. HDAC inhibition also results in an overall decrease in MeCP2 levels of different cell lines. Moreover, we show that miR132 increases upon TSA treatment, and is one of the players involved in the observed downregulation of MeCP2. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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