| Autor: |
Kada, Seoussen, Bouriche, Hamama, Senator, Abderrahmane, Demirtaş, Ibrahim, Özen, Tevfik, Çeken Toptanci, Bircan, Kızıl, Göksel, Kızıl, Murat |
| Předmět: |
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| Zdroj: |
Pharmaceutical Biology; Dec2017, Vol. 55 Issue 1, p330-337, 8p |
| Abstrakt: |
Context:Hertia cheirifoliaL. (Asteraceae), a perennial shrub widely distributed in Northern Africa, is traditionally used to treat inflammatory disorders. Objective:The protective effect of methanol (Met E) and aqueous (Aq E) extracts ofHertia cheirifoliaagainst DNA, lipid and protein oxidation was investigated. Materials and methods:Different concentrations (50–1000 μg/mL) ofHertia cheirifoliaaerial part extracts were examined against DNA, lipid and protein oxidation induced by H2O2 + UV, FeSO4, and Fe3+/H2O2-ascorbic acid, respectively. The DPPH•, metal ion chelating, reducing power and β-carotene bleaching tests were conducted. Results:Both extracts were rich in polyphenols, flavonoids and tannins, and were able to scavenge DPPH•with IC50values of 138 and 197 μg/mL, respectively. At 300 μg/mL, Aq E exerted stronger chelating effect (99%) than Met E (69%). However, Met E reducing power (IC50 = 61 μg/mL) was more than that of Aq E (IC50 = 193 μg/mL). Both extracts protected from β-carotene bleaching by 74% and 94%, respectively, and inhibited linoleic acid peroxidation. The inhibitory activity of Aq E extract (64%) was twice more than that of Met E (32%). Interestingly, both extracts protected DNA against the cleavage by about 96–98%. At 1 mg/mL, Met E and Aq E restored protein band intensity by 94–99%. Conclusions:Hertia cheirifoliaexhibits potent antioxidant activity and protects biomolecules against oxidative damage; hence, it may serve as potential source of natural antioxidant for pharmaceutical applications and food preservation. This is the first report on the protective activity of this plant against biomolecule oxidation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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