| Autor: |
Barnewall, Roy E., Riffle, Carol G., Jones, Randy L., Guistino, David J., Chou, Richard M., Anderson, Mike S., Vassar, Michelle L., Howland, Carrie A. |
| Zdroj: |
Journal of Biochemical & Molecular Toxicology; Dec2017, Vol. 31 Issue 12, pn/a-N.PAG, 12p |
| Abstrakt: |
Ricin toxin may be used as a biological warfare agent and no medical countermeasures are currently available. Here, a well-characterized lot of ricin was aerosolized to determine the delivered dose for future pre-clinical efficacy studies. Mouse intraperitoneal (IP) median lethal dose (LD50) bioassay measured potency at 5.62 and 7.35 μg/kg on Days 0 and 365, respectively. Additional analyses included total protein, sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, and rabbit reticulocyte lysate activity assay. The nebulizer aerosol produced consistent concentrations (2.5 × 103, 5.0 × 103, 1.0 × 104, and 1.5 × 104 μg/mL) and spray factor values. The aerosol particle size distribution was of sufficient size to deposit in lung alveoli (1.12-1.43 μm). Ricinus communis Agglutinin II (RCA 60), prepared at 19 mg/mL in phosphate-buffered saline, pH 7.8, and stored at −70°C, maintained attributes for toxicity following 1-year storage and aerosolized consistently. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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