| Autor: |
Hassan, Abdulkalig Babiker, Suliman, Mohamed Al-Mogbel, Bashir, Abdelhafiz Ibrahim, Shadeed, Mamoun, Ibrahim, Nasir A., Ahmed, Mohammed Qumani, Alaraj, Mohamed Ibrahim |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Oct2017, Vol. 17 Issue 2, p761-767, 7p |
| Abstrakt: |
In this study, the effect of royal jelly on the nephrotoxicity of Gentamicin in the kidneys of female rats was investigated by evaluation of serum indices and histopathological analyses. Thirty female Wistar albino rats were randomly divided into five groups; Group (1) negative control (NC) inoculated intraperitoneally IP with NS for 10 days; Group (2) Royal Jelly (RJ) 50 mg/kg body weight was given orally for 10 days; Group (3) Gentamicin (Gen) 100 mg/kg inoculated IP for 10 days served as positive control; Group (4) RJ + Gen was given royal jelly orally at a dose of 50 mg/kg body weight for 10 days and thereafter, 100 mg/kg Gen was administered IP for 10 days; and Group (5) Gen + RJ was inoculated IP with Gen for 10 days as a dosage of 100 mg/kg and thereafter, was given RJ orally as a dose of 50 mg/kg body weight. Gentamicin has a significant effect on serum urea and creatinine where urea in positive control (Gen) group found to be significantly higher (242±53.5) in comparison to negative control (NC) group (58±4.0). The effect of gentamicin on serum urea was almost nullified when royal jelly was given after gentamycin (43±4.0) while royal jelly given before gentamicin slightly lowers urea but the effect is not statistically significant (209±41.7). Royal jelly alone has no significant effect on serum urea (35±4.4). Almost the same was observed regarding the creatinine in positive control (Gen) group was found to be significantly higher (2.41±0.455) in comparison to negative control (NC) group (0.47±0.005). The effect of gentamicin on serum creatinine was almost nullified when royal jelly was given after gentamicin (0.61±0.053) while royal jelly given before gentamicin slightly lowers creatinine but the effect is not statistically significant (1.67±0.238). Royal jelly alone has no significant effect on serum creatinine (0.47±0.005). Histopathological sections revealed gentamicin group 3 to exert massive degeneration of the tubular epithelium with presence of eosinophilic hyaline cast in the lumen plus dilatation of the renal tubules. In addition there was thickening of the basement membrane of the glomeruli and congestion of the glomerular tuft with sometimes detachment and disappearance of the tubular epithelium of the glomerular membrane. Regarding the groups of royal jelly given before or after gentamicin; there was dilatation of the renal tubules showed some of the tubules to be filled with eosinophilic hyaline material plus thickening of the basement membrane of the glomeruli, vacuolation of the glomerular epithelium, capillary congestion, degeneration of the tubular epithelium when gentamicin was given after royal jelly, this is nearly similar to PC group. The picture was seemed to be better when royal jelly has been given after gentamicin where it was showed no significant pathological changes in glomeruli and tubular epithelium compared to PC group. Royal jelly alone showed wide spread of dilation of renal tubules without significant pathological changes of tubules. No significant effect in the serum indices for uric acid, total protein and albumin in all groups. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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