| Autor: |
Hussein, Nadheema H., Taha, Buthainah M., Abdu-Allah, Shahrazad Najem, Hussein, Jumaah D. |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Oct2017, Vol. 17 Issue 2, p635-638, 4p |
| Abstrakt: |
One of the essential measures of hospital infection control is the use of disinfectants for inanimate objects and surfaces. For this purpose, the present study aims to investigated genotypic of antiseptic-resistance qacA/B gene in clinical and environmental isolates of Staphylococcus hominis subsp. hominis. Twenty-five clinical and environmental Staphylococcus hominis subsp. hominis (S. hominis subsp. hominis) isolates were isolated then itidentified at species level by Vitek-2 system by ID-GP cards and molecular study implemented by PCR technique. Out of 25 Staphylococcus hominis subsp. hominis isolates, 16(64%) isolates were from blood specimens and 9(36%) isolates were from hospital environmental specimens. The results of antibiotic resistance patterns of 25 S. hominis subsp. hominis isolates revealed that all isolates had 100% resistance to Benzylpenicillin and Oxacillin flowed by erythromycin (80%). Also the isolates showed a highest sensitivity rate to Linezolid and Tigecycline (100%) flowed by vancomycin (92%), Teicoplanin, Moxifloxacin and Nitrofurantion (88%). The results of Polymerase Chain Reaction (PCR) assay to detect the presence of qacA/B gene among the 25 S. hominis subsp. hominis isolates under study showed the presence of qacA/B gene (361-bp) in 7(28%) isolates (isolates number 1, 5, 7, 11, 14, 18 and 20), while the other 18(72%) S. hominis subsp. Hominis isolates were not carried qacA/B gene. qacA/B Genein this study strongly suggested that S. hominis subsp. hominis isolates harbor qacA/B Gene, which it is responsible of antiseptic-resistance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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