| Autor: |
Blackman, S. A., Obendorf, R. L., Leopold, A. C. |
| Předmět: |
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| Zdroj: |
Physiologia Plantarum; Apr95, Vol. 93 Issue 4, p630-638, 9p |
| Abstrakt: |
The consistent correlation between desiccation tolerance in orthodox seed tissue and an accumulation of certain “late embryogenesis abundant” (LEA) proteins suggests that these proteins reduce desiccation-induced cellular damage. The aim of the present work was to test this hypothesis. Exogenous abscisic acid (ABA) was used to elevate the level of heat-soluble LEA-like proteins in axes from immature (30 days after flowering: mid-development) seeds of soybean (Glycine max [L.] Merrill cv. Chippewa 64). As the LEA-like proteins accumulated in response to ABA, the leakage of all elements after desiccation and subsequent rehydration markedly declined. Both LEA-like protein accumulation and the decline in desiccation-induced electrolyte leakage were apparently dependent on the presence of ABA. Both effects of ABA were inhibited by cycloheximide. Light microscopy revealed a marked effect of the ABA on cellular integrity following desiccation. Osmotic stress also caused a decrease in desiccation-induced electrolyte leakage and stimulated the accumulation of LEA-like proteins. Our data are consistent with the hypothesis that the LEA-like proteins contribute to the increase in desiccation tolerance in response to ABA, and are consistent with a general protective role for these proteins in desiccation tolerance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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