| Autor: |
Wang, J., Letham, D. S., Taverner, E., Badenoch-Jones, J., Hocart, C. H. |
| Předmět: |
|
| Zdroj: |
Physiologia Plantarum; Sep95, Vol. 95 Issue 1, p91-98, 8p |
| Abstrakt: |
Cytokinins occur in a diversity of forms and determination of their individual levels requires extensive purification. However, determination of the total level of each major base in free, riboside and nucleotide forms would often be adequate. Hence, a methanolysis procedure which releases cytokinin bases from 9‐ribosyl derivatives was developed and applied to plant extracts. A simple procedure, involving low pressure column chromatography, for purification of the cytokinin bases in treated extracts, and a scintillation proximity immunoassay for their quantification, were developed. The total level of each cytokinin base [N6‐(2‐isopentenyl)adenine, zeatin and dihydrozeatin] in free and ribosylated forms determined by these methods is reported for several plant tissues and the results are compared with those obtained after additional purification by HPLC. Values for zeatin were not changed by HPLC but isopentenyl‐adenine and dihydrozeatin levels were usually reduced indicating the presence of unknown compounds which cross‐react in the immunoassay. Modifications to the above purification method to quantify O‐glucosyl cytokinins are also described. The methods described facilitate the quantification of the total amount of each cytokinin base in forms closely associated with cytokinin action, and the detection of cytokinin biosynthesis by labelled precursor incorporation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text