PAR-1 and PAR-2 Expression Is Enhanced in Inflamed Odontoblast Cells.
| Autor: | Alvarez, M. M. P., Moura, G. E., Machado, M. F. M., Viana, G. M., de Souza Costa, C. A., Tjäderhane, L., Nader, H. B., Tersariol, I. L. S., Nascimento, F. D. |
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| Předmět: |
PROTEASE-activated receptors
GENE expression ODONTOBLASTS DENTAL pulp DENTAL caries G protein coupled receptors N-terminal residues POLYMERASE chain reaction GENETICS PHYSIOLOGY RNA metabolism BIOCHEMISTRY CELL receptors CONNECTIVE tissue cells HIGH performance liquid chromatography INFLAMMATION MASS spectrometry PHENOMENOLOGY MICROSCOPY PROTEOLYTIC enzymes |
| Zdroj: | Journal of Dental Research; Dec2017, Vol. 96 Issue 13, p1518-1525, 8p, 5 Graphs |
| Abstrakt: | Protease-activated receptors (PARs) are G protein-coupled receptors, which are activated by proteolytical cleavage of the amino-terminus and act as sensors for extracellular proteases. We hypothesized that PAR-1 and PAR-2 can be modulated by inflammatory stimulus in human dental pulp cells. PAR-1 and PAR-2 gene expression in human pulp tissue and MDPC-23 cells were analyzed by quantitative polymerase chain reaction. Monoclonal PAR-1 and PAR-2 antibodies were used to investigate the cellular expression of these receptors using Western blot, flow cytometry, and confocal microscopy in MDPC-23 cells. Immunofluorescence assays of human intact and carious teeth were performed to assess the presence of PAR-1 and PAR-2 in the dentin-pulp complex. The results show for the first time that human odontoblasts and MDPC-23 cells constitutively express PAR-1 and PAR-2. PAR-2 activation increased significantly the messenger RNA expression of matrix metalloproteinase (MMP)-2, MMP-9, MMP-13, and MMP-14 in MDPC-23 cells ( P < 0.05), while the expression of these enzymes decreased significantly in the PAR-1 agonist group ( P < 0.05). The high-performance liquid chromatography and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis showed the presence of MMP-13 activity cleaving PAR-1 at specific, noncanonical site TLDPRS42↓F43LL in human dental pulp tissues. Also, we detected a presence of a trypsin-like activity cleaving PAR-2 at canonical site SKGR20↓S21LIGRL in pulp tissues. Confocal microscopy analysis of human dentin-pulp complex showed intense positive staining of PAR-1 and PAR-2 in the odontoblast processes in dentinal tubules of carious teeth compared to intact ones. The present results support the hypothesis of activation of the upregulated PAR-1 and PAR-2 by endogenous proteases abundant during the inflammatory response in dentin-pulp complex. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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