| Autor: |
Pourfathi, Mehrdad, Xin, Yi, Kadlecek, Stephen J., Cereda, Maurizio F., Profka, Harrilla, Hamedani, Hooman, Siddiqui, Sarmad M., Ruppert, Kai, Drachman, Nicholas A., Rajaei, Jennia N., Rizi, Rahim R. |
| Zdroj: |
Magnetic Resonance in Medicine; Dec2017, Vol. 78 Issue 6, p2106-2115, 10p |
| Abstrakt: |
Purpose To investigate pulmonary metabolic alterations during progression of acute lung injury. Methods Using hyperpolarized [1-13C] pyruvate imaging, we measured pulmonary lactate and pyruvate in 15 ventilated rats 1, 2, and 4 h after initiation of mechanical ventilation. Lung compliance was used as a marker for injury progression. 5 untreated rats were used as controls; 5 rats (injured-1) received 1 ml/kg and another 5 rats (injured-2) received 2 ml/kg hydrochloric acid (pH 1.25) in the trachea at 70 min. Results The mean lactate-to-pyruvate ratio of the injured-1 cohort was 0.15 ± 0.02 and 0.15 ± 0.03 at baseline and 1 h after the injury, and significantly increased from the baseline value 3 h after the injury to 0.23 ± 0.02 ( P = 0.002). The mean lactate-to-pyruvate ratio of the injured-2 cohort decreased from 0.14 ± 0.03 at baseline to 0.08 ± 0.02 1 h after the injury and further decreased to 0.07 ± 0.02 ( P = 0.08) 3 h after injury. No significant change was observed in the control group. Compliance in both injured groups decreased significantly after the injury ( P < 0.01). Conclusions Our findings suggest that in severe cases of lung injury, edema and hyperperfusion in the injured lung tissue may complicate interpretation of the pulmonary lactate-to-pyruvate ratio as a marker of inflammation. However, combining the lactate-to-pyruvate ratio with pulmonary compliance provides more insight into the progression of the injury and its severity. Magn Reson Med 78:2106-2115, 2017. © 2017 International Society for Magnetic Resonance in Medicine. [ABSTRACT FROM AUTHOR] |
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