MicroRNA-155-3p Mediates TNF-α-Inhibited Cementoblast Differentiation.
| Autor: | Wang, X., Sun, H., Liao, H., Wang, C., Jiang, C., Zhang, Y., Cao, Z. |
|---|---|
| Předmět: |
MICRORNA
TUMOR necrosis factors PERIODONTITIS PERIODONTIUM BIOMINERALIZATION CEMENTUM RNA metabolism ANIMAL experimentation CELL culture CELL differentiation CELL lines CELLULAR signal transduction FLUORESCENT antibody technique MICE POLYMERASE chain reaction PROTEINS RNA WESTERN immunoblotting FETAL development REMINERALIZATION (Teeth) |
| Zdroj: | Journal of Dental Research; Nov2017, Vol. 96 Issue 12, p1430-1437, 8p, 1 Diagram, 4 Graphs |
| Abstrakt: | Periodontitis is a prevalent and chronic inflammatory disease that is interrelated with systemic health. Periodontitis can be promoted by tumor necrosis factor α (TNF-α). Cementum, a vital part of the periodontium, is a bone-like mineralized tissue that is produced by cementoblasts. Our laboratory previously revealed that TNF-α inhibits cementoblast differentiation and mineralization. However, how TNF-α modulates cementoblast differentiation and mineralization remains largely unknown. MicroRNA-155 (miR-155) is induced and regulates TNF-α-inhibited osteogenic differentiation. In this study, we found that miR-155-3p was increased during TNF-α-stimulated OCCM-30 cells and involved in cementoblast differentiation and mineralization. Overexpression of miR-155-3p suppressed cementoblast mineralization. Bioinformatics analysis revealed that potassium channel tetramerization domain containing 1 ( Kctd1) is a candidate target gene of miR-155-3p. Moreover, miR-155-3p overexpression suppressed KCTD1 levels. Meanwhile, its knockdown increased KCTD1 expression. Transfection with miR-155-3p also inhibited the luciferase activity of 3'-untranslated regions in the Kctd1 wild type but not the mutant. These data indicated that Kctd1 is a direct and novel target of miR-155-3p. The Wnt signaling pathway inhibits cementoblast differentiation, and we further demonstrated that miR-155-3p partially modulates cementoblast differentiation through the canonical Wnt signaling pathway. In addition to the gain/loss function assay of miR-155-3p, the luciferase activity assay of canonical Wnt signaling was performed. The assays revealed that miR-155-3p increased β-catenin-mediated transcriptional activation. Overall, our data clarified that miR-155-3p mediated TNF-α-inhibited cementoblast differentiation by targeting Kctd1, at least partially through canonical Wnt signaling pathway. These findings reveal the expanded function of miRNAs in cementoblast differentiation and mineralization. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
| Externí odkaz: |
|