| Autor: |
Alonso, Mariana, Melani, Mariana, Converso, Daniela, Jaitovich, Ariel, Paz, Cristina, Carreras, M. Cecilia, Medina, Jorge H., Poderoso, Juan J. |
| Předmět: |
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| Zdroj: |
Journal of Neurochemistry; 4/1/2004, Vol. 89 Issue 1, p248-256, 9p |
| Abstrakt: |
Intracellular activation and trafficking of extracellular signal-regulated protein kinases (ERK) play a significant role in cell cycle progression, contributing to developmental brain activities. Additionally, mitochondria participate in cell signalling through energy-linked functions, redox metabolism and activation of pro- or anti-apoptotic proteins. The purpose of the present study was to analyze the presence of ERK1/2 in mitochondria during rat brain development. Immunoblotting, immune electron microscopy and activity assays demonstrated that ERK1/2 are present in fully active brain mitochondria at the outer membrane/intermembrane space fraction. Besides, it was observed that ERK1/2 translocation to brain mitochondria follows a developmental pattern which is maximal between E19-P2 stages and afterwards declines at P3, just before maximal translocation to nucleus, and up to adulthood. Most of mitochondrial ERK1/2 were active; upstream phospho-MAPK/ERK kinases (MEK1/2) were also detected in the brain organelles. Mitochondrial phospho-ERK1/2 increased at 1 μ m hydrogen peroxide (H2O2 ) concentration, but it decreased at higher 50–100 μ m H2O2 , almost disappearing after the organelles were maximally stimulated to produce H2O2 with antimycin. Our results suggest that developmental mitochondrial activation of ERK1/2 cascade contributes to its nuclear translocation effects, providing information about mitochondrial energetic and redox status to the proliferating/differentiating nuclear pathways. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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