Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer.

Autor:	Tang, Shan, Liang, Lu ‐ Jun, Si, Yan ‐ Yan, Gao, Shuai, Wang, Jia ‐ Xing, Liang, Jun, Mei, Ziqing, Zheng, Ji ‐ Shen, Liu, Lei
Předmět:	UBIQUITIN ISOMER synthesis ISOPEPTIDE bonds COST effectiveness UBIQUITINATION
Zdroj:	Angewandte Chemie; 10/16/2017, Vol. 129 Issue 43, p13518-13522, 5p
Abstrakt:	Chemical ubiquitination is an effective approach for accessing structurally defined, atypical ubiquitin (Ub) chains that are difficult to prepare by other techniques. Herein, we describe a strategy that uses a readily accessible premade isopeptide-linked 76-mer (isoUb), which has an N-terminal Cys and a C-terminal hydrazide, as the key building block to assemble atypical Ub chains in a modular fashion. This method avoids the use of auxiliary-modified Lys and instead employs the canonical and therefore more robust Cys-based native chemical ligation technique. The efficiency and capacity of this isoUb-based strategy is exemplified by the cost-effective synthesis of several linkage- and length-defined atypical Ub chains, including K27-linked tetra-Ub and K11/K48-branched tri-, tetra-, penta-, and hexa-Ubs. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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