Down regulation of protective genes is associated with cellular and antibody-mediated rejection.

Autor: Lin‐Wang, Hui Tzu, Cipullo, Reginaldo, Dinkhuysen, Jarbas J., Finger, Marco A., Rossi, João M., Correia, Edileide B., Hirata, Mário H.
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Zdroj: Clinical Transplantation; Oct2017, Vol. 31 Issue 10, pn/a-N.PAG, 8p
Abstrakt: Despite advances in immunosuppressive therapy, rejection still remains the main obstacle to a successful transplant. This study aims to explore the gene expression profile of the rejection process in order to decrease the number of unnecessary endomyocardial biopsies in stable patients. Methods A total of 300 formalin-fixed and paraffin-embedded ( FFPE) endomyocardial biopsies sampled from 63 heart allograft recipients were included in this study. Acute cellular rejection ( ACR) and antibody-mediated rejection ( AMR) were diagnosed by histological analysis and immunohistochemical C4d staining, respectively. Analysis of gene expression was performed by quantitative real-time polymerase chain reaction. The samples were grouped according to the ISHLT rejection classification, aiming the statistical analysis. Results There was a significant decrease in the HMOX1, AIF1, and CCL2 transcript over the post-transplantation period in non-rejection group ( P<.001). Furthermore, the ADIPOR1, ADIPOR2, BCL2L1, and VEGFA protective genes were significantly downregulated in the ACR group ( P<.05). ADIPOR2, BCL2L1, IL6, and NOS2 genes were also significantly downregulated in the AMR group than in the non-rejection group ( P<.05). Conclusion The downregulations of the protective genes contribute to the allograft rejection, and the archived FFPE samples are useful for the gene expression analysis aiming the allograft rejection surveillance. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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