Response Surface Methodology Optimization of Thebaine Biotransformation into Codeine and Morphine Using Bacillus sp. FAR.

Autor: Ataee, Nazanin, Fooladi, Jamshid, Namaei, Mohammad Hasan, Mirzajani, Fateme
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Zdroj: Indian Journal of Pharmaceutical Education & Research; Oct-Dec2017, Vol. 51 Issue 4, p684-691, 8p
Abstrakt: Background: Codeine and morphine are important pharmaceuticals having analgesic characteristics. Aim: The current study used response surface methodology to optimize the biotransformation of thebaine into codeine and morphine using Bacillus sp. FAR. Methods: A central composite design was used to determine the optimal concentrations of buffer and thebaine, pH, time, temperature, and biomass. Improvement in transformation was monitored using HPLC-DAD. Results: The optimized conditions employed were pH 7.7 at 36°C for 30 h using a thebaine concentration of 80 µg/mL, 45 mM tris of buffer and 2.9% w/v biomass. The optimal conditions for codeine and morphine production were 2.995 and 1.113 µg, respectively. Conclusion: Optimization demonstrated that pH and biomass had the strongest effects on biotransformation. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index