| Autor: |
Ruiz-Miyazawa, Kenji, Staurengo-Ferrari, Larissa, Mizokami, Sandra, Domiciano, Talita, Vicentini, Fabiana, Camilios-Neto, Doumit, Pavanelli, Wander, Pinge-Filho, Phileno, Amaral, Flávio, Teixeira, Mauro, Casagrande, Rubia, Verri, Waldiceu |
| Předmět: |
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| Zdroj: |
Inflammopharmacology; Oct2017, Vol. 25 Issue 5, p555-570, 16p |
| Abstrakt: |
We investigated the anti-inflammatory and analgesic effects of quercetin in monosodium urate crystals (MSU)-induced gout arthritis, and the sensitivity of quercetin effects to naloxone, an opioid receptor antagonist. Mice were treated with quercetin, and mechanical hyperalgesia was assessed at 1-24 h after MSU injection. In vivo, leukocyte recruitment, cytokine levels, oxidative stress, NFκB activation, and gp91 and inflammasome components (NLRP3, ASC, Pro-caspase-1, and Pro-IL-1β) mRNA expression by qPCR were determined in the knee joints at 24 h after MSU injection. Inflammasome activation was determined, in vitro, in lipopolysaccharide-primed macrophages challenged with MSU. Quercetin inhibited MSU-induced mechanical hyperalgesia, leukocyte recruitment, TNFα and IL-1β production, superoxide anion production, inflammasome activation, decrease of antioxidants levels, NFκB activation, and inflammasome components mRNA expression. Naloxone pre-treatment prevented all the inhibitory effects of quercetin over MSU-induced gout arthritis. These results demonstrate that quercetin exerts analgesic and anti-inflammatory effect in the MSU-induced arthritis in a naloxone-sensitive manner. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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