| Autor: |
Eastcott, Jean W., Taubman, Martin A., Smith, Daniel J., Holt, Stanley C. |
| Předmět: |
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| Zdroj: |
Oral Microbiology & Immunology; Feb1990, Vol. 5 Issue 1, p8-11, 4p |
| Abstrakt: |
The purpose of this investigation was to determine the component(s) of whole Actinobacillus actinomycetemcomitans bacteria responsible for B cell mitogenic activity. Congenitally athymic "nude" rats were used as a source of B cells devoid of T lymphocyte activity. Spleen cells were cultured with, or without, whole formalin-killed A. actinomycetemcomitans bacteria or with purified LPS from A. actinomycetemcomitans. Dose-response curves to A. actinomycetemcomitans cells or to A. actinomycetemcomitans-LPS showed that responses were dose dependent. If optimal quantities of both A. actinomycetemcomitans and A. actinomycetemcomitans-LPS were added in combination, the proliferative responses were the same as if either was added alone, i.e., the responses were not additive Polymyxin B at 2μg/well completely abrogated the proliferative response of athymic rat splenocytes to 107 A. actinomycetemcomitans cells or to 1.25 μg A. actinomycetemcomitans-LPS/well. Therefore, the in vitro early proliferative response of B cells to A. actinomycetemcomitans can be attributed to the presence of LPS on A. actinomycetemcomitans cells A considerable portion of the in situ lymphocytic gingival response to A.actinomycetemcomitans infection seen in periodontal disease patients may be a B cell mitogenic response to the LPS of this bacterium [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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