| Autor: |
Martins da Costa, Elaine, Azarias Guimarães, Amanda, Pereira Vicentin, Rayssa, Almeida Ribeiro, Paula, Ribas Leão, Aniele, Balsanelli, Eduardo, Lebbe, Liesbeth, Aerts, Maarten, Willems, Anne, Souza Moreira, Fatima |
| Předmět: |
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| Zdroj: |
Archives of Microbiology; Oct2017, Vol. 199 Issue 8, p1211-1221, 11p |
| Abstrakt: |
Four strains of rhizobia isolated from nodules of Vigna unguiculata (UFLA03-321, UFLA03-320 and UFLA03-290) and Macroptilium atropurpureum (UFLA04-0212) in Brazilian soils were previously reported as a new group within the genus Bradyrhizobium. To determine their taxonomic position, these strains were characterized in this study using a polyphasic approach. The analysis of the 16S rRNA gene grouped the four strains with Bradyrhizobium pachyrhizi PAC48. However, the concatenated sequence analysis of the two ( recA and glnII) or three ( atpD, gyrB and recA) housekeeping genes indicated that these strains represent a novel species of Bradyrhizobium, which is very closely related to B. pachyrhizi PAC48 and B. elkanii USDA 76. Genomic relatedness analyses between the UFLA03-321 strain and B. elkanii USDA 76 and B. pachyrhizi PAC48 revealed an average nucleotide identity below 96% and values of estimated DNA-DNA hybridization below 70%, confirming that they represent genomically distinct species. Analysis of MALDI-TOF MS (Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry) profiles and phenotypic characteristics also allowed differentiation of the novel species from its two neighboring species. In phylogenetic analysis of nodC and nifH genes, UFLA03-321 exhibited maximum similarity with B. tropiciagri CNPSo 1112. The data suggest that these four UFLA strains represent a novel species, for which the name Bradyrhizobium brasilense sp. nov. is proposed, with UFLA03-321 (=LMG 29353 =CBAS645) as type strain. G + C content in the DNA of UFLA03-321 is 63.9 mol %. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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