Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering.

Autor: Granados, M., Morticelli, L., Andriopoulou, S., Kalozoumis, P., Pflaum, M., Iablonskii, P., Glasmacher, B., Harder, M., Hegermann, J., Wrede, C., Tudorache, I., Cebotari, S., Hilfiker, A., Haverich, A., Korossis, Sotirios
Zdroj: Journal of Cardiovascular Translational Research; Aug2017, Vol. 10 Issue 4, p374-390, 17p
Abstrakt: Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% ( w/ v) SDS and 0.5% ( w/ v) SD and sterilized with 0.1% ( v/ v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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