Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay.

Autor: Kazakova, A. S., Imatdinov, I. R., Dubrovskaya, O. A., Imatdinov, A. R., Sidlik, M. V., Balyshev, V. M., Krasochko, P. A., Sereda, A. D.
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Zdroj: Transboundary & Emerging Diseases; Oct2017, Vol. 64 Issue 5, p1479-1492, 14p
Abstrakt: This article is devoted to the development and evaluation of the immunoblotting test system for serological diagnosis of African swine fever ( ASF), based on the highly purified recombinant p30 of ASF virus ( ASFV) strain Stavropol 01/08 (Stavropol 2008), representative of the ASFV currently circulating in the Russian Federation. The main project stages are as follows: (i) cloning of the central hydrophilic region of the ASFV gene CP204L (p30) into a prokaryotic vector; (ii) expression and chromatographic purification of the recombinant product p30 with thioredoxin and poly-histidine site (p30e1_TrxA_6xHis); (iii) development of the immunoblotting test system (Rec p30- IB) using the highly purified recombinant p30; and (iv) evaluation of Rec p30- IB using sera and organ samples from domestic pigs and wild boars experimentally or naturally infected by ASFV. Testing of the Rec p30- IB showed the diagnostic specificity and sensitivity of the assay to be 98.75% and 100.00%, respectively. High sensitivity of the Rec p30- IB allowed the detection of ASFV-specific antibodies in samples of organs of the immune system and blood sera, collected from domestic pigs and wild boars, starting from 6 to 8 days post-infection, regardless of virus virulence, seroimmunotype and geographic origin of the samples (East Europe, South Europe, West Europe, Central and south-east Africa). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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