Comparison of haemostatic function of PAS-C-platelets vs. plasma-platelets in reconstituted whole blood using impedance aggregometry and thromboelastography.

Autor: Hout, F. M. A., Bontekoe, I. J., Laleijne, L. A. E., Kerkhoffs, J. ‐ L., Korte, D., Eikenboom, J., Bom, J. G., Meer, P. F.
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Zdroj: Vox Sanguinis; Aug2017, Vol. 112 Issue 6, p549-556, 8p, 4 Charts, 1 Graph
Abstrakt: Background and Objectives There are concerns about the haemostatic function of platelets stored in platelet additive solution ( PAS). Aim of this study was to compare the haemostatic function of PAS-C-platelets to plasma-platelets in reconstituted whole blood. Materials and Methods In our experiment, whole blood was reconstituted with red blood cells, solvent-detergent ( SD) plasma and either PAS-C-platelets or plasma-platelets ( n = 7) in a physiological ratio. On storage days 2, 5, 8 and 13, the agonist-induced aggregation (multiple electrode aggregometry), clot formation (thromboelastography) and agonist-induced CD62P responsiveness (flow cytometry) were measured. Results Samples with PAS-C-platelets showed significantly lower aggregation than plasma-platelets when induced with adenosine diphosphate, −6 U (95% confidence interval: −8; −4) or thrombin receptor-activating protein, −15 U (−19; −10). Also when activated with collagen and ristocetin, the PAS-C-platelets showed less aggregation, although not statistically significant. All samples with PAS-C-platelets showed significantly lower agonist-induced CD62P responsiveness than samples with plasma-platelets. However, there was no difference regarding all TEG parameters. Conclusion Our findings demonstrate that the function - aggregation and CD62P responsiveness - of PAS-C-platelets in reconstituted whole blood is inferior to that of plasma-platelets, which may have implications in the setting of massive transfusions. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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