| Autor: |
Bei Xue, Pei Zhang, Li, Zhi H., Lian Zhao, Xu, Wan Y., Sun, Jin Q., Ma, Hang K., Yan, Bin L., Huan Gao |
| Předmět: |
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| Zdroj: |
Journal of Crustacean Biology; Jul2017, Vol. 37 Issue 4, p398-405, 8p |
| Abstrakt: |
The periodic shedding of the exoskeleton is an essential physiological process for muscle growth and postembryonic development in crustaceans. The physiology and biochemistry of skeletal muscles in the caridean prawn Palaemon carinicauda (Holthuis, 1950), a cultured species, are nevertheless poorly understood. To investigate the expression profiles of myosin heavy chain (MHC), we cloned and characterized both the full-length cDNA (PcMHC) and the genomic DNA sequence (dPcMHC) of MHC in P. carinicauda. The full-length cDNA of PcMHC was 6184 bp, including a 5742 bp open reading frame (ORF) encoding 1913 amino acids without a signal peptide. The tissue distribution patterns of PcMHC showed the highest expression in muscle compared to other tissues. The expression levels of PcMHC were higher in individuals at different post-molt time points, compared to the inter-molt period, reaching a peak 5 min after molting and then decreasing. This suggests that PcMHC may be involved in muscle remodeling after molting. The genomic DNA and the 5'-flanking sequence of MHC were also isolated by genome walking. To investigate whether or not the fluctuating expression levels of PcMHC were regulated by DNA methylation, three pairs of bisulfite sequencing PCR (BSP) primers, located at upstream of the transcription start site (TSS) to the exon 2, were collected to analyze the methylation profile of dPcMHC at different post-molt time points. We found that dPcMHC of both inter-molt and post-molt individuals were un-methylated, suggesting that the up-regulation of PcMHC might not be adjusted by DNA methylation in the regions we detected. We suggest PcMHC to be an "early" isoform, and that "late" isoforms may also exist in P. carinicauda. Additional studies are clearly needed to understand the mechanisms of muscle development during molt in crustaceans. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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