| Autor: |
Lazarus, Lawrence H., Itin, Ahuva, Popescu, Mircea, Goldblum, Natan |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 1972, Vol. 27 Issue 2, p335-340, 6p |
| Abstrakt: |
Replicase induced by foot-and-mouth disease virus has an absolute requirement for Mg2+ that cannot be replaced by other divalent cations. Mn2+ and Ca2+ are effective non-competitive inhibitors. At low ionic strength, Mg2+ directly influence the V of the reaction. High ionic strength (0.2 M KCI), produces a shift in the optimum Mg2+ concentration toward lower values which enhances the V and Km. KCI is more active in stimulating activity than NaCI, LiCI or CsCI but similar to NH4CI. (NH4),2SO4, however. is less stimulatory than the chloride salt. K+ appear to interact specifically with replicase so as to affect initiation: once the reaction has been initiated it becomes refractory to the addition of K+ and shows typical plateau kinetics under all conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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